二抗、标记二抗选择指南

二抗是与一抗来源不同的，宿主体内制备的，能与一抗或一抗片段结合的抗体。它可通过信号放大提高灵敏度，并为标记和检测提供了更大的灵活性，通常标记酶或荧光素等行使功能。

二抗可以耦联不同的标记物，如酶（辣根过氧化酶HRP和碱性磷酸酶AP或其衍生物APAAP，PAP），荧光基团（FITC, PE, Cy荧光素, BF荧光素）, 生物素和胶体金等。

标记二抗具有高特异性、高灵敏度等性质，被广泛用于免疫学实验中，选用哪种偶联物标记的二抗取决于具体的实验。

识别物种：Rabbit
二抗名称	未标记	现货标记物
Goat anti-Rabbit IgG	bs-0295G	Biotin, AP, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF568, BF594, BF647, BF680, BF700, BF750, Cy3, Cy5, Cy5.5, Cy7, PE, APC, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCP-Cy5.5, Gold, SA
Donkey anti-Rabbit IgG	bs-0295D	Biotin, AP, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF594, BF647, BF680, BF750, Cy3, Cy5, Cy5.5, Cy7, PE, APC, PE-Cy5, PE-Cy7, Gold
AffiniPure F(ab')₂ Fragment Goat anti-Rabbit IgG (H+L)	bs-60295G	Biotin, HRP, FITC, BF488
Mouse anti-Rabbit IgG (H + L)	bs-0295M	Biotin, AP, HRP, FITC, RBITC, BF488, BF555, BF594, BF647, BF750, Cy3, Cy5, Cy5.5, PE, APC, PE-Cy5, PE-Cy7, Gold
Mouse anti-Rabbit IgM	bs-0369M	Biotin, AP, HRP, FITC, RBITC, BF350, BF555, Cy3, Cy5, PE, APC, PE-Cy7, Gold
Mouse anti-Rabbit IgG monoclonal antibody	bsm-0295M	HRP, Gold
识别物种：Mouse
二抗名称	未标记	现货标记物
Goat anti-Mouse IgG	bs-0296G	Biotin, AP, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF594, BF647, BF680, BF750, Cy3, Cy5, Cy5.5, Cy7, PE, APC, PE-Cy5, PE-Cy5.5, PE-Cy7, Gold, SA
Rabbit anti-Mouse IgG	bs-0296R	Biotin, AP, HRP, FITC, RBITC, BF350, BF488, BF555, BF594, BF647, Cy3, Cy5, Cy7, PE, APC, PE-Cy5.5, PE-Cy7, Gold
Rabbit anti-Mouse Kappa light chain	bs-0330R	AP, HRP, FITC, BF488, BF647, Cy7, PE, APC, Gold
Goat anti-Mouse IgM	bs-0368G	Biotin, AP, HRP, FITC, RBITC, BF350, BF488, BF555, BF594, BF647, Cy3, Cy5, Cy5.5, Cy7, PE, APC, PE-Cy5, PE-Cy7, Gold
Rabbit anti-Mouse IgM	bs-0368R	HRP, FITC, RBITC, BF488, BF555, BF594, BF647, Cy3, Cy5, PE, APC, Gold
Rabbit anti-Mouse IgG-Fc	bs-0377R	Biotin, HRP, FITC, BF488, BF555, BF647, Cy5, PE, APC
AffiniPure F(ab')₂ Fragment Goat anti-Mouse IgG (H+L)	bs-60296G	Biotin, HRP, FITC, PE, APC
Rat anti-Mouse IgD	bsm-34233A
识别物种：Human
二抗名称	未标记	现货标记物
Donkey anti-Human IgG	bs-0297D	Biotin, AP, HRP, FITC, BF488, BF555, BF647, BF750, Cy3, Cy5, PE, APC, Gold
Goat anti-Human IgG	bs-0297G	Biotin, AP, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF594, BF647, BF680, Cy3, Cy5, Cy5.5, PE, APC, PE-Cy7, Gold
Mouse anti-Human IgG	bs-0297M	Biotin, AP, HRP, FITC, RBITC, BF594, BF647, Cy3, Cy5, Cy5.5, PE, APC, PE-Cy5, Gold
Rabbit anti-Human IgG	bs-0297R	Biotin, AP, HRP, FITC, RBITC, BF405, BF488, BF555, BF647, Cy3, Cy5, PE, APC, PE-Cy5, Gold
Goat anti-Human IgM	bs-0345G	Biotin, AP, HRP, FITC, BF488, Cy3, Cy5, PE, APC, Gold
Mouse anti-Human IgM	bs-0345M	Biotin, HRP, FITC, Cy5, Gold
Rabbit anti-Human IgM	bs-0345R	Biotin, AP, HRP, FITC, BF555, BF647, Cy3, PE, APC, Gold
Rabbit anti-Human IgA	bs-0360R	Biotin, AP, HRP, FITC, BF647, Cy3, PE, APC, Gold
Rabbit anti-Human IgG F (ab')2	bs-0364R	Biotin, AP, HRP, FITC, BF488, Cy5, Cy5.5, PE, APC
Mouse anti-Human IgG monoclonal antibody	bsm-0297M	Biotin, AP, HRP, FITC, RBITC, BF405, BF488, BF555, BF594, BF647, BF680, BF700, BF750, Cy5, PE, AE, APC, PerCP, PE-Cy5.5, PE-Cy7, APC-Cy5.5, APC-Cy7, PerCP-Cy5.5, PerCP-Cy7, PacificBlue, IRDye800CW
Mouse anti-Human IgM	bsm-41524M
Mouse anti-Human IgG mAb	bsm-41525M
Goat anti Human IgE	bs-2226G	AP, HRP, FITC, Cy3, PE
Human IgG Fc	bs-0365R	HRP, APC
Human IgG Fc	bsm-0365M	AP, HRP, RBITC, PE, APC
识别物种：Rat
二抗名称	未标记	现货标记物
Goat anti-Rat IgG	bs-0293G	Biotin, AP, HRP, FITC, RBITC, BF350, BF488, BF555, BF594, BF647, Cy3, Cy5, Cy7, PE, APC, PE-Cy5.5, PE-Cy7, Gold
Mouse anti-Rat IgG	bs-0293M	Biotin, AP, HRP, FITC, BF350, BF488, BF647, Cy3, Cy5.5, APC, Gold
Rabbit anti-Rat IgG	bs-0293R	Biotin, AP, HRP, FITC, RBITC, BF350, BF488, BF555, BF594, BF647, Cy3, Cy5, PE, APC, PE-Cy5, PE-Cy7, Gold
Rabbit anti-Rat IgM	bs-0346R	Biotin, HRP, FITC, BF350, BF488, BF555, BF647, Cy3, Cy7, PE, APC, PE-Cy7
识别物种：Goat
二抗名称	未标记	现货标记物
Donkey anti-Goat IgG	bs-0294D	Biotin, AP, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF594, BF647, BF680, BF750, Cy3, Cy5, Cy7, PE, APC, PE-Cy5, PE-Cy7, Gold
Mouse anti-Goat IgG	bs-0294M	Biotin, AP, HRP, FITC, RBITC, BF488, BF555, BF594, Cy3, Cy5, Cy5.5, PE, Gold
Rabbit anti-Goat IgG	bs-0294R	Biotin, AP, HRP, FITC, RBITC, BF350, BF488, BF555, BF594, BF647, Cy3, Cy5, PE, APC, PE-Cy5, Gold
Rabbit anti-Goat IgM	bs-0370R	Biotin, AP, HRP, FITC, BF488, Cy3, Cy5, Cy5.5, PE
Mouse anti Goat IgG monoclonal antibody	bsm-0294M
识别物种：Bovine
二抗名称	未标记	现货标记物
Goat anti-Bovine IgG	bs-0326G	Biotin, HRP, FITC, BF488, BF647, PE-Cy5.5, PE-Cy7, Gold
Mouse anti-Bov IgG	bs-0326M	HRP, FITC, BF488, Cy5, PE-Cy7, Gold
Rabbit anti-Bov IgG	bs-0326R	AP, HRP, FITC, BF488, APC, Gold
Rabbit anti-Bovine IgM	bs-0327R	Biotin, HRP, FITC, BF488, BF647, PE
识别物种：Chicken
二抗名称	未标记	现货标记物
Goat anti-Chicken IgG	bs-0310G	Biotin, AP, HRP, FITC, RBITC, BF405, BF488, BF555, BF594, BF647, Cy3, Cy5, Cy7, PE, APC, Gold
Rabbit anti-Chicken IgG	bs-0310R	Biotin, AP, HRP, FITC, RBITC, BF488, BF594, BF647, Cy3, PE, APC, Gold
Rabbit anti-Chicken IgM	bs-0314R	AP, HRP, FITC, BF488, Cy3, APC, Gold
Goat anti-chicken IgY H&L	bs-0432G	FITC, BF488
Mouse anti-Chicken IgY	bs-0432M
Rabbit anti-Chicken IgY	bs-0432R	Biotin, HRP, RBITC, BF350, BF488, BF555, BF594, Cy3, Cy7
Mouse anti-Chicken IgG H&L	bs-0310M
识别物种：Dog
二抗名称	未标记	现货标记物
Rabbit anti-Dog IgG	bs-0303R	Biotin, AP, HRP, FITC, Cy5, PE, APC, PE-Cy7, Gold
Rabbit anti-Dog IgM	bs-0373R	HRP, FITC, RBITC, BF488, Cy3, PE-Cy7, Gold
识别物种：Guinea pig
二抗名称	未标记	现货标记物
Donkey anti-Guinea pig IgG	bs-0358D	Biotin, AP, HRP, FITC, RBITC, BF488, BF555, BF594, BF647, BF750, Cy3, Cy5, Gold
Goat anti-Guinea pig IgG	bs-0358G	Biotin, HRP, FITC, RBITC, BF350, BF405, BF488, BF555, BF647, Cy3, PE, APC
Mouse anti-Guinea pig IgG	bs-0358M	HRP, RBITC, BF647
Rabbit anti-Guinea pig IgG	bs-0358R	Biotin, HRP, FITC, Cy3, Gold
Rabbit anti-Guinea pig IgM	bs-0375R	HRP, FITC, RBITC, BF488, BF594
识别物种：Cat
二抗名称	未标记	现货标记物
Rabbit anti-Cat IgG H&L	bs-10749R	HRP, BF488
识别物种：Horse
二抗名称	未标记	现货标记物
Rabbit anti-Horse IgG	bs-0308R	Biotin, AP, HRP, FITC, RBITC, BF488, Cy3, Cy5, PE, APC, Gold
识别物种：Mongolian Gerbil
二抗名称	未标记	现货标记物
Rabbit anti-MG IgG	bs-0403R	Biotin, HRP, FITC, Cy5
识别物种：Mink
二抗名称	未标记	现货标记物
Rabbit anti-Mink IgG	bs-0777R	HRP, FITC, Cy5, PE-Cy5
识别物种：Monkey
二抗名称	未标记	现货标记物
Rabbit anti-Monkey IgG	bs-0335R	Biotin, AP, HRP, FITC, BF555, BF647, APC, Gold
Rabbit anti-Monkey IgM	bs-0336R	Biotin, HRP, FITC
识别物种：Pig
二抗名称	未标记	现货标记物
Rabbit anti-Pig IgG H&L	bs-0309R	Biotin, AP, HRP, FITC, RBITC, BF488, BF647, Cy3, Cy5.5, Cy7, PE, APC, Gold
识别物种：Avidin
二抗名称	未标记	现货标记物
Rabbit anti-Avidin	bs-0312R	FITC, Cy5, Gold
识别物种：Biotin
二抗名称	未标记	现货标记物
Rabbit anti-Biotin	bs-0311R	Biotin, AP, HRP, FITC, RBITC, BF405, BF488, BF555, BF594, BF647, BF680, BF700, BF750, Cy3, Cy5, Cy5.5, PE, AE, APC, PerCP, PE-Cy5, PE-Cy5.5, PE-Cy7, APC-Cy5.5, APC-Cy7, PerCP-Cy5.5, PerCP-Cy7, PacificBlue, Gold