BF488标记山羊抗兔IgG(H+L), F(ab')2片段抗体

Target: Rabbit
Host: Goat
Antibody Format: F(ab')? Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Human, Mouse, Rat Serum Proteins
Conjugate: Alexa Fluor? 488 (Amax: 493 Emax: 519nm)
Product Category: F(ab')? Fragment Affinity-Purified Antibodies
Dilution Range: 1:100-800 for most applications
Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Information:
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species.

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.