The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells.
产品应用
应用
已检合格种属
预测种属
推荐稀释比例
WB
Mouse, Rat
Human
1:500-2000
IHC-P
Human, Mouse, Rat
1:100-500
IHC-F
Human, Mouse, Rat
1:100-500
IF
Human, Mouse, Rat
1:100-500
交叉反应
交叉反应: Human, Mouse, Rat
相关产品
暂无相关产品
靶标
基因名
ENPP3
蛋白名
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
亚细胞定位
Membrane; Single-pass type II membrane protein (Potential). Secreted. Note=Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
组织特异性
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
翻译后修饰
N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
相似性
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
功能
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.