eIF4EBP1 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF,IP | Bioss

bsm-63191R

重组兔单抗

eIF4EBP1 Recombinant Rabbit mAb

eIF4E结合蛋白重组兔单抗

4E-BP1; 4EBP1; BP-1; PHAS-I; 4EBP1_HUMAN; EIF4EBP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I); 4EBP1_MOUSE; 4EBP1_RAT; eukaryotic translation initiation factor 4E binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1

Rabbit

A synthesized peptide derived from human 4E BP1: 1-46

IgG

Liquid

affinity purified by Protein A

Recombinant

9F8

13 kDa

13-16 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    4E-BP1是一种分子量为10-12kDa 的酸性蛋白，与eIF4G 竞争结合。4E-BPs 对 eIF4E 的结合是可逆的，并依赖于 4E-BP 的磷酸化状态。非磷酸化的4E-BP1 能与 eiF4E 牢固结合，而磷酸化的4E-BP1则不能。
而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通过对35、45、69位的苏氨酸残基或64位的丝氨酸残基进行磷酸化而使4E-BP1丧失对eIF4E结合能力的激酶。然而，并非所有的磷酸化事件都能同样地阻断4EBP1-eIF4E的相互作用。

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

EIF4EBP1

Eukaryotic translation initiation factor 4E-binding protein 1

Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.

Nucleus.

Lung and testis.

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1.

Amyloidosis 5 (AMYL5) [MIM:105120]: A hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. Note=The disease is caused by mutations affecting the gene represented in this entry.

Belongs to the eIF4E-binding protein family.

Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.