CDKN2A/p16-INK4a Rabbit pAb (一抗) - IHC-P,IHC-F,IF | Bioss
货号：bs-23797R
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概述
产品编号
bs-23797R
产品类型
病理级抗体、衰老相关抗体、农牧业/家禽抗体
英文名称
CDKN2A/p16-INK4a Rabbit pAb
中文名称
抑癌基因p16抗体
英文别名
ARF; CAI2; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16; ARF-INK4a; INK4a-ARF; Ink4a/Arf; Pctr1; p16(INK4a); p19; p16Cdkn2a; CDN2A_HUMAN; CDKN2A; Cyclin-dependent kinase 4 inhibitor A (CDK4I); Multiple tumor suppressor 1 (MTS-1); p16-INK4a (p16-INK4 | p16INK4A); CDN2A_MOUSE; p16-INK4a (p16-INK4); CDN2A_RAT; p16-INK4a (p16 | p16-INK4);
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human CDKN2A/p16-INK4a: 61-156/156
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
17 kDa
检测分子量
17 kDa
浓度
1mg/ml
储存液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域
Cancer > Cell cycle > Cell cycle inhibitors > Ink4
Cancer > Oncoproteins/suppressors > Tumor suppressors > p53 pathway
Cell Biology > Cell Cycle > Cell Cycle Inhibitors > Ink4
Epigenetics and Nuclear Signaling > Cell cycle > Cell Cycle Inhibitors > Ink4
Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
p16主要功能是通过抑制CDK4而阻止细胞由G1期进入S期，使细胞增殖受到限制。用于各种恶性肿瘤如肺癌、恶黑、乳腺癌的研究。目前的研究细胞周期依赖激酶抑制p16INK4a蛋白在宫颈上皮内病变(CIN)中作为一个新标记物. p16INK4a的过表达与HPV E7区(病毒早期蛋白即病毒致癌基因编码区)活性有密切相关性。 p16/CDKN2基因是新近发现的肿瘤抑制基因，已有研究表明该基因在许多肿瘤出现缺失、突变或重排现象.
背景资料
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012].
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| IHC-P | Human, Mouse, Rat | Pig | 1:100-500 |
| IHC-F | Human, Mouse, Rat | Pig | 1:100-500 |
| IF | Human, Mouse, Rat | Pig | 1:100-500 |
交叉反应
交叉反应: Human, Mouse, Rat (predicted: Pig)
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靶标
基因名
Cdkn2a
蛋白名
Tumor suppressor ARF
亚基
Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts (isoforms 1,2 and 4) with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2.
亚细胞定位
Cytoplasm. Nucleus.
组织特异性
Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.
翻译后修饰
Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation.
疾病
Note=The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.
Defects in CDKN2A are the cause of cutaneous malignant melanoma type 2 (CMM2) [MIM:155601]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719].
Defects in CDKN2A are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. [DISEASE] Defects in CDKN2A are the cause of melanoma-astrocytoma syndrome (MASTS) [MIM:155755]. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma.
Defects in CDKN2A are the cause of cutaneous malignant melanoma type 2 (CMM2) [MIM:155601]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719].
Defects in CDKN2A are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. [DISEASE] Defects in CDKN2A are the cause of melanoma-astrocytoma syndrome (MASTS) [MIM:155755]. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma.
相似性
Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Contains 4 ANK repeats.
Contains 4 ANK repeats.
功能
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
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