Recombinant HPV16 E7 protein, DsbC & His (重组蛋白) | Bioss
货号：bs-49101P
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概述
产品编号
bs-49101P
英文名称
Recombinant HPV16 E7 protein, DsbC & His
中文名称
重组人类乳头状瘤病毒16-E7蛋白
英文别名
E7; HPV16 E7 protein; Human Papilloma Virus; Human papillomavirus type 16 E7; Human papillomavirus type 16 E7; Protein E7; Human Papillomavirus 16 (E7); HPV16 E7; E7 protein (HPV16); VE7_HPV16; HPV16-E7; Recombinant HPV16 E7 protein, DsbC & His
性状
Lyophilized or Liquid
纯化方法
AC
理论分子量
41
浓度
＞0.5 mg/ml
储存液
20mM Tris-HCl (pH8.0) with 150mM NaCL.
研究领域
活性
Not tested
序列
1-98/98
标签
DsbC & His
纯度
>95% as determined by SDS-PAGE
内毒素
Not analyzed
表达系统
E.coli
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
保存条件
Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍
E7/E6protein抗体主要用于性病及妇科肿瘤方面的研究，HPV16是宫颈癌的主要致癌病毒，也用于与HPV有关的各种肿瘤的研究。表达位置：胞核。阳性细胞也可见于角蛋白层，尤其是在疣中。该抗体用于人类生殖系统传染性疾病及其肿瘤的研究，亦可用于食管癌、乳腺癌等恶性肿瘤的研究。
背景资料
Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.
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靶标
基因名
E7
蛋白名
HPV16 Protein E7
亚基
Homodimer. Homooligomer. Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity. Binds to CHD3 through its zinc-finger domain. Forms a complex with CHD3 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD3 and HDAC2 might also form. Interacts with E2; this interaction inhibits E7 oncogenic activity.
亚细胞定位
Host cytoplasm. Host nucleus.
翻译后修饰
Phosphorylated by host ERK. The phosphorylation triggers a structural change that enhances keratin binding and protein stability.
相似性
Belongs to the papillomaviridae E7 protein family.
功能
E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription.