CLOCK Recombinant Rabbit mAb (一抗) - IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF | Bioss

bsm-54050R

重组兔单抗

CLOCK Recombinant Rabbit mAb

生物钟基因hClock重组兔单抗

KAT13D; bHLHe8; 5330400M04Rik; CLOCK_HUMAN; CLOCK; hCLOCK; Class E basic helix-loop-helix protein 8 (bHLHe8); 2.3.1.48; KIAA0334; CLOCK_MOUSE; mCLOCK; CLOCK_RAT; rCLOCK; clock circadian regulator; clock (mouse) homolog; clock homolog (mouse); Circadian locomoter output cycles protein kaput

Rabbit

A synthesized peptide derived from human CLOCK: 145-180

IgG

Liquid

affinity purified by Protein A

Recombinant

3F9

95 kDa

95 kDa

1mg/ml

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CLOCK

Circadian locomoter output cycles protein kaput

Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif

Cytoplasm. Nucleus. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus.

Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation.

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL.