EPAS1 | HIF2A Recombinant Rabbit mAb (一抗) - WB,Flow-Cyt,ICC/IF | Bioss

bsm-54278R

重组兔单抗

EPAS1 | HIF2A Recombinant Rabbit mAb

缺氧诱导因子2α/EPAS1重组兔单抗

ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-2alpha; HRF; EPAS; EPAS-1; HIF-2 alpha; HIF2 alpha; EPAS1_HUMAN; EPAS1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73 (bHLHe73); HIF-1-alpha-like factor (HLF); Hypoxia-inducible factor 2-alpha (HIF-2-alpha | HIF2-alpha); Member of PAS protein 2; PAS domain-containing protein 2; EPAS1_MOUSE; HIF-1-alpha-like factor (HLF | mHLF); HIF-related factor (HRF); EPAS1_RAT; endothelial PAS domain protein 1; HIF-1 alpha-like factor

Rabbit

A synthesized peptide derived from human HIF 2 alpha: 52-108

IgG

Liquid

affinity purified by Protein A

Recombinant

2G3

96 kDa

120 kDa

1mg/ml

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

HIF-2α也是机体对缺氧适应性调节中的重要调控蛋白.有学者认为：HIF-2α对肿瘤的能量代谢和新血管生成有促进作用,微血管的形成可抑制肿瘤细胞的凋亡,从而促进肿瘤的恶性发展。

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

EPAS1

Endothelial PAS domain-containing protein 1

Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBPB.

Nucleus (Potential).

Expressed in most tissues, with highest levels in lung, followed by heart, kidney, brain and liver. Predominantly expressed in endothelial cells. Also found in smooth muscle cells of the uterus, neurons, and brown adipose tissue. High expression in embryonic choroid plexus and kidney glomeruli.

In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.