G3BP1 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF,IP | Bioss
Rrmab?兔单抗
货号：bsm-62130R
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概述
产品编号
bsm-62130R
产品类型
重组兔单抗
英文名称
G3BP1 Recombinant Rabbit mAb
中文名称
Ras GTP酶活化蛋白结合蛋白1重组兔单抗
英文别名
G3BP; HDH-VIII; G3BP1_HUMAN; G3BP1; G3BP-1; ATP-dependent DNA helicase VIII (hDH VIII); GAP SH3 domain-binding protein 1; 3.6.4.12; 3.6.4.13; G3BP stress granule assembly factor 1; GTPase activating protein (SH3 domain) binding protein 1; Rasputin homolog; Ras-GTPase-activating protein SH3-domain-binding protein; DNA helicase VIII
抗体来源
Rabbit
免疫原
A synthesized peptide derived from human G3BP1: 401-466/466
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Recombinant
克隆号
18C17
理论分子量
52 kDa
检测分子量
65 kDa
储存液
10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
研究领域
SWISS
Gene ID
保存条件
Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料
Protein involved in various processes, such as stress granule formation and innate immunity.Plays an essential role in stress granule formation.Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress.Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations.
基因名
G3BP1
蛋白名
Ras GTPase-activating protein-binding protein 1
亚基
Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity). Interacts with USP10, and may regulate it. Forms homodimers and oligomers.
亚细胞定位
Cytoplasm. Cytoplasm, cytosol. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells. Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
组织特异性
Ubiquitous.
翻译后修饰
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
相似性
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 RRM (RNA recognition motif) domain.
功能
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human, Mouse, Rat | 1:1000-2000 | |
| IHC-P | Human, Mouse, Rat | 1:100-500 | |
| IHC-F | Human, Mouse, Rat | 1:100-500 | |
| IF | Human, Mouse, Rat | 1:100-500 | |
| Flow-Cyt | Human | Mouse, Rat | 1μg/Test |
| ICC/IF | Human | Mouse, Rat | 1:50-200 |
| IP | Human | Mouse, Rat | 1:50-200 |
交叉反应
交叉反应: Human, Mouse, Rat
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