胰高血糖素重组兔单抗

概述

产品编号

bsm-62053R

产品类型

重组兔单抗

英文名称

Glucagon Recombinant Rabbit mAb

中文名称

胰高血糖素重组兔单抗

英文别名

GLP-1; GLP1; GLP2; GRPP; Glu; PPG; GLUC_HUMAN; GCG; GLUC_MOUSE; GLUC_RAT;

抗体来源

Rabbit

免疫原

A synthesized peptide derived from human Glucagon: 53-73

亚型

IgG

性状

Liquid

纯化方法

affinity purified by Protein A

克隆类型

Recombinant

克隆号

4G7

理论分子量

21

检测分子量

21

储存液

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

SWISS

P01275

Gene ID

2641

保存条件

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

数据库链接

Entrez Gene : 2641 Human

Entrez Gene : 14526 Mouse

Entrez Gene : 24952 Rat

Omim : 138030 Human

SwissProt : P01275 Human

SwissProt : P55095 Mouse

SwissProt : P06883 Rat

Unigene : 516494 Human

Unigene : 45494 Mouse

Unigene : 54383 Rat

产品介绍

产品组分及规格

编号	组分	规格	浓度	储存
1	PBS缓冲液（干粉）	2 L×2	20x	室温
2	抗原修复缓冲液	20 ml	100x	2-8℃
3	内源性过氧化物酶阻断剂	3 ml	RTU	2-8℃
4	封闭工作液	3 ml	RTU	2-8℃
5	一抗（Rat GLP-1 Mouse mAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Mouse IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit（20×）显色液	0.3 ml	RTU	-20℃
8	DAB kit（20×）稀释液	0.3 ml	RTU	-20℃
9	复染试剂	5 ml	RTU	室温
10	封片剂	5 ml	RTU	室温
11	对照切片（大鼠胰腺）	1张	RTU	室温
12	说明书	1份

背景

GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。

2. 抗原修复

沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体，加入2-4滴封闭工作液（试剂4），置于湿盒内37℃封闭20 min，以减少非特异性染色。

5. 一抗孵育

用吸水纸吸去玻片上多余的液体，加入2-4滴大鼠GLP-1鼠单抗工作液（试剂5），置于湿盒中，4℃孵育过夜或37℃孵育1-2 h。

6. 复温

4℃孵育过夜后，室温下复温15 min (若在室温下孵育一抗，则直接进入下一步清洗)；用PBS洗涤5 min，重复3次。

7. 二抗孵育

用吸水纸吸玻片上多余的液体，加入2-4滴HRP标记羊抗鼠IgG工作液（试剂6），置于湿盒中，37℃孵育1-2 h；用PBS洗涤5 min，重复3次。

8. 显色

用吸水纸吸去玻片上多余的液体，在每张切片上滴加约50 μL新配制的DAB工作液（试剂7:试剂8:PBS=1:1:18），作用3-5 min。显微镜下观察结果，达到合适的显色强度后，用蒸馏水冲洗切片以终止反应，用蒸馏水冲洗5 min，重复3次。

9. 复染

滴加适量复染试剂（试剂9）复染3-5 min，蒸馏水冲洗5 min，滴加盐酸酒精分化约30 s，蒸馏水洗涤5 min，重复2次。

10. 脱水封片

将玻片依次置于不同浓度（70%、80%、90%、95%、100%）乙醇，各5 min；然后置于新鲜二甲苯中浸泡脱蜡3次，每次15 min。用吸水纸吸去多余的二甲苯，滴加适量封片剂（试剂10）在组织上，将盖玻片盖在组织上，避免产生气泡。

注意事项

1. 建议检测时进行阴性及阳性对照，以提高实验的可靠性。

2. 本品中的配套试剂，请不要用其他生产商产品替换使用。

3. DAB为致癌物质，请采取必要的防范措施。

4. PBS洗涤液（试剂1）配制后在4℃可保存一周；抗原修复液（试剂2）及显色剂（试剂7和8）的工作液需每次实验时现用现配。

*5. 发表论文时引用本产品的写作建议 "IHC0109R, Bioss Antibodies"。引用示例: “Rat tissue sections using Rat GLP-1 IHC Kit (IHC0109R, Bioss Antibodies) were stained for GLP-1 according to the manufacturer's instructions.”

背景资料

Glucagon: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.

产品应用

应用	已检合格种属	预测种属	推荐稀释比例
WB	Mouse	Human, Rat	1:500-2000
IHC-P	Human, Mouse, Rat		1:100-500
IHC-F	Human, Mouse, Rat		1:100-500
IF	Human, Mouse, Rat		1:100-500

交叉反应

交叉反应: Human, Mouse, Rat

靶标

基因名

GCG

蛋白名

Pro-glucagon

亚基

Secreted.

亚细胞定位

Secreted.

组织特异性

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.

翻译后修饰

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

相似性

Belongs to the glucagon family.

功能

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis.
GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life

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胰高血糖素重组兔单抗

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