Dnmt1 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,ICC/IF | Bioss

bsm-61399R

重组兔单抗

Dnmt1 Recombinant Rabbit mAb

DNA甲基转移酶1重组兔单抗

DNMT1_BOVIN; DNMT1; 2.1.1.37; DNMT1_HUMAN; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI (DNA MTase HsaI | M.HsaI); MCMT; AIM; CXXC9; DNMT; DNMT1_MOUSE; Met-1; DNA methyltransferase MmuI (DNA MTase MmuI | M.MmuI); Met1; Uim; DNMT1_RAT; DNA MTase RnoIP (M.RnoIP); DNA methyltransferase I; DNA methyltransferase 1; DNA (cytosine-5-)-methyltransferase 1

Rabbit

A synthesized peptide derived from human DNMT1: 1500-1616

IgG

Liquid

affinity purified by Protein A

Recombinant

1B3

183 kDa

220 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

DNA甲基转移酶-1在多种肿瘤细胞中表达量相当高，而在正常成人细胞中则低表达，因此dnmt1基因的高表达与肿瘤的发生有密切的关系。 dnmt-1 对细胞周期、增殖及凋亡有一定的的影响。细胞核表达。 (isoform CRA_a)为抑制细胞增殖、促进细胞凋亡，为肿瘤的基因治疗提供依据。

DNMT1

DNA(cytosine-5)-methyltransferase 1

Binds to CSNK1D (By similarity). Homodimer. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with UBC9 and BAZ2A/TIP5.

Nucleus.

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Sumoylated; sumoylation increases activity.
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.

Defects in DNMT1 are the cause of hereditary sensory neuropathy type 1E (HSN1E) [MIM:614116]. A neurodegenerative disorder characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia.

Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.