PKM2 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF | Bioss

bsm-61301R

重组兔单抗、mIHC精品抗体

PKM2 Recombinant Rabbit mAb

丙酮酸激酶M2重组兔单抗

CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1; p58; Pk-2; Pk-3; PK; PKM12; KPYM_HUMAN; PKM; Cytosolic thyroid hormone-binding protein (CTHBP); Opa-interacting protein 3 (OIP-3); Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1 (THBP1); Tumor M2-PK; Tyrosine-protein kinase PKM2; 2.7.1.40; PK2; KPYM_MOUSE; Pykm; KPYM_RAT; pyruvate kinase M1/2; pyruvate kinase, muscle

Rabbit

A synthesized peptide derived from human PKM: 50-100

IgG

Liquid

affinity purified by Protein A

Recombinant

9H2

58 kDa

60 kDa

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

丙酮酸激酶（PK）有L、R、M1、M2四种同功酶，均为四聚体。 L型主要分布于肝脏，R型存在于红细胞，在结构、免疫学和动力学上十分相似，由同一基因调控；M1存在于肌肉中，M2分布于除上述以外的其他组织（尤以肾脏最多）以及胎儿各组织。PK也是一种癌胚蛋白，在恶性肿瘤中，增高的都是M2型。

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production.

PKM

Pyruvate kinase PKM

Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia.

Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

ISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.

Belongs to the pyruvate kinase family.

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.