线粒体促凋亡蛋白重组兔单抗

概述

产品编号

bsm-61284R

产品类型

重组兔单抗

英文名称

Smac Recombinant Rabbit mAb

中文名称

线粒体促凋亡蛋白重组兔单抗

英文别名

DFNA64; SMAC; 0610041G12Rik; 1700006L01Rik; DBLOH_HUMAN; DIABLO; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspases (SMAC); DBLOH_MOUSE; Second mitochondria-derived activator of caspase (Smac);

抗体来源

Rabbit

免疫原

A synthesized peptide derived from human DIABLO: 200-239/239

亚型

IgG

性状

Liquid

纯化方法

affinity purified by Protein A

克隆类型

Recombinant

克隆号

8E4

理论分子量

27 kDa

检测分子量

21 kDa

储存液

10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

SWISS

Q9NR28

Gene ID

56616

保存条件

Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

数据库链接

Entrez Gene : 56616 Human

Entrez Gene : 66593 Mouse

Entrez Gene : 288753 Rat

Omim : 605219 Human

SwissProt : Q9NR28 Human

SwissProt : Q9JIQ3 Mouse

Unigene : 169611 Human

Unigene : 46716 Mouse

产品介绍

Smac（second mitochondria-derived activator of caspase）又称DIABLO（direct IAP binding protein），是一种线粒体蛋白，也是重要的促细胞凋亡蛋白之一，能够促进细胞凋亡，当杀死不正常细胞的机制启动后，线粒体释放出Smac蛋白促使受损或不正常细胞凋亡的生化过程就会出现，Smac在诱导肿瘤细胞凋亡，增加肿瘤细胞的化疗敏感性及增强免疫力等方面有一定的意义和应用前景。Smac是第二个线粒体来源的胱氨酸酶激活剂，它通过拮抗或中和一种或几种凋亡抑制蛋白（IAP）家族成员而发挥其促凋亡作用。

背景资料

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases.

产品应用

应用	已检合格种属	预测种属	推荐稀释比例
WB	Human, Mouse, Rat		1:500-2000
IHC-P	Human, Mouse, Rat		1:50-200
IHC-F	Human, Mouse, Rat		1:50-200
IF	Human, Mouse, Rat		1:50-200
Flow-Cyt	Human	Mouse, Rat	1:50-100
ICC/IF		Human, Mouse, Rat	1:50-200
IP		Human, Mouse, Rat	1:20-50

交叉反应

交叉反应: Human, Mouse, Rat

靶标

基因名

DIABLO

蛋白名

Diablo homolog, mitochondrial

亚基

Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.

亚细胞定位

Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.

组织特异性

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

翻译后修饰

Ubiquitinated by BIRC7/livin.

疾病

Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry.

功能

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

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线粒体促凋亡蛋白重组兔单抗

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交叉反应

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