ATF4 Rabbit pAb (一抗) | Bioss

2026-03-02~2026-04-30,KXJ26032026-03-02~2026-04-30,促销赠品
ATF4 Rabbit pAb (一抗) | Bioss
货号:bs-1531R
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概述

产品编号
bs-1531R
产品类型
宠物抗体、农牧业/家禽抗体
英文名称
ATF4 Rabbit pAb
中文名称
活化转录因子4抗体
英文别名
CREB-2; CREB2; TAXREB67; TXREB; Atf-4; C/ATF; ATF4_HUMAN; ATF4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2 (CREB-2 | cAMP-responsive element-binding protein 2); Tax-responsive enhancer element-binding protein 67 (TaxREB67); Q96AQ3_HUMAN; ATF4_MOUSE; C/EBP-related ATF (C/ATF); Q5U4B2_MOUSE; Q8CF69_MOUSE; ATF4_RAT; Activating transcription factor 4 (rATF-4);
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human ATF4: 251-351/351
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
38 kDa
检测分子量
47 kDa
浓度
1mg/ml
储存液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料
ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
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产品应用

应用已检合格种属预测种属推荐稀释比例
WBHuman, Mouse, RatRabbit, Pig, Sheep, Cow, Dog, Horse1:500-2000
IHC-PHuman, Mouse, RatRabbit, Pig, Sheep, Cow, Dog, Horse1:100-500
IHC-FMouseHuman, Rat, Rabbit, Pig, Sheep, Cow, Dog, Horse1:100-500
IFHuman, Mouse, RatRabbit, Pig, Sheep, Cow, Dog, Horse1:100-500
Flow-CytHuman, MouseRat, Rabbit, Pig, Sheep, Cow, Dog, Horse0.2μg /test
ICC/IFHumanMouse, Rat, Rabbit, Pig, Sheep, Cow, Dog, Horse1:100-500

交叉反应

交叉反应: Human, Mouse, Rat (predicted: Rabbit, Pig, Sheep, Cow, Dog, Horse)

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靶标

基因名
ATF4
蛋白名
Cyclic AMP-dependent transcription factor ATF-4
亚基
Binds DNA as a homo- or heterodimer. Interacts (via its leucine zipper domain) with GABBR1 and GABBR2 (via their C-termini). Interacts (via its DNA binding domain) with FOXO1 (C-terminal half); the interaction occurs in osteoblasts and regulates glucose homeostasis through suppression of beta-cell proliferation and a decrease in insulin production. Interacts with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors. Interacts with CEP290 (via an N-terminal region). Interacts with NEK6, DAPK2 (isoform 2) and ZIPK/DAPK3. Forms a heterodimer with TXLNG in osteoblasts. Interacts with DDIT3/CHOP.
亚细胞定位
Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
翻译后修饰
Ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4.
Phosphorylated by NEK6. Phosphorylated on the betaTrCP degron motif at Ser-219, followed by phosphorylation at Thr-213, Ser-224, Ser-231, Ser-235 and Ser-248, promoting interaction with BTRC and ubiquitination. Phosphorylation is promoted by mTORC1.
Phosphorylated by NEK6.
相似性
Belongs to the bZIP family.
Contains 1 bZIP domain.
功能
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.

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