MUC1 | CA 15-3 Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF | Bioss

bsm-55523R

重组兔单抗、mIHC精品抗体

MUC1 | CA 15-3 Recombinant Rabbit mAb

粘蛋白-1重组兔单抗

ADMCKD; ADMCKD1; ADTKD2; CA 15-3; CD227; Ca15-3; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM; MUC1_HUMAN; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3 (CA 15-3); Carcinoma-associated mucin; Episialin; Krebs von den Lungen-6 (KL-6); Peanut-reactive urinary mucin (PUM); Polymorphic epithelial mucin (PEM); Tumor-associated epithelial membrane antigen (EMA); Tumor-associated mucin; MUC1_MOUSE; mucin 1, cell surface associated; mucin 1, transmembrane; medullary cystic kidney disease 1 (autosomal dominant); epithelial membrane antigen

Rabbit

Synthesized peptide derived from Human MUC1

IgG

Liquid

Affinity Purification

Recombinant

SB48

350 kDa

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Mucin（CD227）是糖蛋白家族的一员，以重复结构域和密集的O-糖基化为特征。MUC1在呼吸道、胃肠道等所有上皮组织、皮脂腺和唾液腺以及造血细胞中都有表达，MUC1的非上皮性表达已被描述发生在白细胞上，包括T细胞、B细胞、骨髓单核细胞、单核细胞和树突状细胞（DCs）等。MUC1可进行蛋白质水解裂解，并且较大的胞外域与较小的羧基末端结构域有关，后者包含一个跨膜片段和一个含72个残基的胞质尾区。MUC1对正常的上皮起润滑和保护作用，同时还介导信号转导和细胞黏附功能，免疫活化和抑制作用等。在肿瘤组织中，MUC1常出现异常表达，非极性分布，且由于糖基转移酶活性增高而导致的糖基化不完全，使正常情况下隐蔽的表位暴露出来，成为肿瘤生物学治疗的靶点。

Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation . MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail. MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation. MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail. Overexpression of MUC1 potentiates transformation and attenuates stress-induced apoptosis through the Akt or p53 pathways

MUC1

Mucin-1

The alpha subunit forms a tight, non-covalent heterodimeric complex with the proteolytically-released beta-subunit.

Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.

Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells.
Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.

Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.

Note=MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.

Contains 1 SEA domain.

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.