phospho-FoxO3a (Ser253) Recombinant Rabbit mAb (一抗) - WB,IHC-P,IHC-F,IF | Bioss

bsm-52156R

磷酸化抗体、重组兔单抗

phospho-FoxO3a (Ser253) Recombinant Rabbit mAb

磷酸化叉头蛋白3A重组兔单抗

AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; 1110048B16Rik; 2010203A17Rik; Fkhr2; FOXO3_HUMAN; FOXO3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3_MOUSE; forkhead box O3; FOXO3 | FoxO3A (phospho-S253); p-FoxO3A; phospho-FoxO3A; p-FOXO3; FOXO3 | FoxO3A (phospho-Ser253)

Rabbit

A synthesized peptide derived from human FOXO3A around the phosphorylation site of S253: AV-pS-MD

IgG

Liquid

affinity purified by Protein A

Recombinant

25B3

71 kDa

100 kDa

1mg/ml

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

FOXO3a是FOX(forkhead box)蛋白家族的一个重要成员,与细胞转化、肿瘤的发生发展及其血管生成有关,是一种已知的控制细胞循环和细胞死亡的蛋白质。

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

FOXO3

Forkhead box protein O3

Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4/MST1, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Interacts with PIM1.

Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Tissue Specificity : Ubiquitous.

Ubiquitous.

In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.

Contains 1 fork-head DNA-binding domain.

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.