phospho-eNOS (Ser1177) Recombinant Rabbit mAb (一抗) - IHC-P,IHC-F,IF | Bioss

bsm-54795R

磷酸化抗体、重组兔单抗

phospho-eNOS (Ser1177) Recombinant Rabbit mAb

磷酸化一氧化氮合成酶3（内皮型）重组兔单抗

ECNOS; eNOS; 2310065A03Rik; Nos-3; NOS3_BOVIN; NOS3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase, endothelial (Endothelial NOS | eNOS); 1.14.13.39; NOS3_HUMAN; NOS3_MOUSE; NOS3_RAT; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); endothelial nitric oxide synthase; NOS3 | eNOS (phospho-S1177); p-eNOS; phospho-eNOS; p-NOS3; NOS3 | eNOS (phospho-Ser1177)

Rabbit

KLH conjugated Synthesised phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177:

IgG

Liquid

affinity purified by Protein A

Recombinant

12H2

133 kDa

1mg/ml

0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

合成与降解（Synthesis and Degradation)
催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。此抗体识别分子量为135kDa的内皮型一氧化氮合成的酶。

NOS3

Nitric oxide synthase, endothelial

Homodimer. Interacts with NOSIP and NOSTRIN.

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Platelets, placenta, liver and kidney.

Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.

Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.