TBP Mouse mAb (一抗) | Bioss
货号：bsm-33227M
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概述
产品编号
bsm-33227M
英文名称
TBP Mouse mAb
中文名称
TATA结合蛋白单克隆抗体
英文别名
GTF2D; GTF2D1; HDL4; SCA17; TBP1; TFIID; TBP_CHICK; TBP; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; TBP_HUMAN; TF2D; TBP_MOUSE;
抗体来源
Mouse
免疫原
Recombinant human TBP protein
亚型
IgG
性状
Size : 50ul/100ul
Liquid
Size : 200ug (PBS only)
Lyophilized
Note: Centrifuge tubes before opening. Reconstitute the lyophilized product in distilled water. Optimal concentration should be determined by the end user.
Liquid
Size : 200ug (PBS only)
Lyophilized
Note: Centrifuge tubes before opening. Reconstitute the lyophilized product in distilled water. Optimal concentration should be determined by the end user.
纯化方法
affinity purified by Protein G
克隆类型
Monoclonal
克隆号
6G8
理论分子量
38 kDa
检测分子量
35-40 kDa
浓度
1mg/ml
储存液
Size : 50ul/100ul
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Size : 200ug (PBS only)
0.01M PBS
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Size : 200ug (PBS only)
0.01M PBS
研究领域
Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies
Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription > TAFs
Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription > TFIIB
Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription > TFIID
Epigenetics and Nuclear Signaling > Transcription > Preinitiation complex
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
在DNA结合多蛋白因子TFIID的核心起作用的一般转录因子。TFIID与TATA盒的结合是pre-initiation complex（PIC）的初始转录步骤，在RNA聚合酶II转录的真核基因激活中起作用。也是一种含有BRF2的转录因子复合物的成分，调节RNA聚合酶III介导的转录。
背景资料
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human | Mouse, Rat | 1:500-1000 |
| IHC-P | Rat | Human, Mouse | 1:100-500 |
| IHC-F | Rat | Human, Mouse | 1:100-500 |
| IF | Rat | Human, Mouse | 1:100-500 |
交叉反应
交叉反应: Human, Rat (predicted: Mouse)
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靶标
基因名
TBP
蛋白名
TATA-box-binding protein
亚基
Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1. Interacts with CITED2 (By similarity). Interacts with ATF7IP. Interacts with HIV-1 Tat.
亚细胞定位
Nucleus.
组织特异性
Widely expressed, with levels highest in the testis and ovary.
疾病
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
相似性
Belongs to the TBP family.
功能
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
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