phospho-SMAD7 (Ser249) Rabbit pAb (一抗) | Bioss
货号：bs-20024R
产品详情
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常见问题
概述
产品编号
bs-20024R
产品类型
磷酸化抗体、农牧业/家禽抗体
英文名称
phospho-SMAD7 (Ser249) Rabbit pAb
中文名称
磷酸化细胞信号转导分子SMAD7抗体
英文别名
SMAD7 (phospho-S249); p-SMAD7; phospho-SMAD7; SMAD7 (phospho-Ser249); Madh7; SMAD7_HUMAN; SMAD7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8 (MAD homolog 8 | Mothers against DPP homolog 8); SMAD family member 7 (SMAD 7 | Smad7 | hSMAD7); MADH8; SMAD7_MOUSE; SMAD family member 7 (SMAD 7 | Smad7); SMAD7_RAT;
抗体来源
Rabbit
免疫原
KLH conjugated synthesised phosphopeptide derived from human SMAD7 around the phosphorylation site of Ser249: SD(p-S)QL
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
46 kDa
检测分子量
50 kDa
浓度
1mg/ml
储存液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
转录调节因子（Transcriptin Regulators）
Smad7是转化生长因子(TGF-β)信号通路的抑制分子，Smad7可干预MAPK信号通路,使ERK和JNK磷酸化活性的平衡失调,导致促增殖作用强于生长抑制作用,从而有助于细胞向恶性方向发展。
Smad7是转化生长因子(TGF-β)信号通路的抑制分子，Smad7可干预MAPK信号通路,使ERK和JNK磷酸化活性的平衡失调,导致促增殖作用强于生长抑制作用,从而有助于细胞向恶性方向发展。
背景资料
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Mouse, Rat | Human, Pig, Cow, GuineaPig | 1:500-2000 |
| IHC-P | Human, Mouse, Rat | Pig, Cow, GuineaPig | 1:100-500 |
| IHC-F | Human, Mouse, Rat | Pig, Cow, GuineaPig | 1:100-500 |
| IF | Human, Mouse, Rat | Pig, Cow, GuineaPig | 1:100-500 |
交叉反应
交叉反应: Human, Mouse, Rat (predicted: Pig, Cow, GuineaPig)
相关产品
暂无相关产品
靶标
基因名
SMAD7
蛋白名
Mothers against decapentaplegic homolog 7
亚基
Interacts with WWP1. Interacts with COPS5. Interacts with NEDD4L. Interacts with STAMBP. Interacts with RNF111, AXIN1 and AXIN2. Interacts with PPP1R15A. Interacts (via MH2 domain) with EP300. Interacts with ACVR1B, SMURF1, SMURF2 and TGFBR1; SMAD7 recruits SMURF1 and SMURF2 to the TGF-beta receptor and regulates its degradation. Interacts with PDPK1 (via PH domain).
亚细胞定位
Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta.
组织特异性
Ubiquitous with higher expression in the lung and vascular endothelium.
翻译后修饰
Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription. Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1.
疾病
Genetic variations in SMAD7 influence susceptibility to colorectal cancer type 3 (CRCS3) [MIM:612229]. Colorectal cancer consists of tumors or cancer of either the colon or rectum or both. Cancers of the large intestine are the second most common form of cancer found in males and females. Symptoms include rectal bleeding, occult blood in stools, bowel obstruction and weight loss. Treatment is based largely on the extent of cancer penetration into the intestinal wall. Surgical cures are possible if the malignancy is confined to the intestine. Risk can be reduced when following a diet which is low in fat and high in fiber.
相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
功能
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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