聚组氨酸抗体标签单克隆抗体

bsm-33004M

标签抗体

His tag Mouse mAb

聚组氨酸抗体标签单克隆抗体

HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.; His Tag;

Mouse

KLH conjugated HHHHHH peptide

IgG

Liquid

affinity purified by Protein G

Monoclonal

6E6

1mg/ml

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

标签抗体
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.