重组鸡新城疫血凝素蛋白
货号：bs-42007P
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概述
产品编号
bs-42007P
英文名称
Recombinant NDV HN protein, His
中文名称
重组鸡新城疫血凝素蛋白
英文别名
hemagglutinin-neuraminidase protein; newcastle disease virus(NDV); HN protein; hemagglutinin-neuraminidase protein [Newcastle disease virus]; HN_NDVB; Newcastle disease virus HN protein; Newcastle disease virus hemagglutinin neuraminidase protein; Recombinant NDV HN protein, His
性状
Lyophilized or Liquid
纯化方法
AC
理论分子量
57.7
检测分子量
65 kDa
浓度
＞0.5 mg/ml
储存液
4M Urea (pH9.0) with 1mM DDT.
研究领域
活性
Not tested
序列
49-577/577
标签
N-His
纯度
>90% as determined by SDS-PAGE
内毒素
Not analyzed
表达系统
E.coli
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
保存条件
Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍
鸡新城疫病毒由副粘病毒引起 鸡新城疫的高度接触性传染病。又称亚洲鸡瘟或伪鸡瘟 。常呈急性败血症状 。主要特征是呼吸困难、便稀、神经紊乱、黏膜和浆膜出血。死亡率高，对养鸡业危害严重。
速发嗜内脏型新城疫(VVND；Doyle，1927)，可致所有年龄的鸡发生最急性或急性、致死性疾病。通常见有消化道出血性病变。
速发嗜肺脑型新城疫(VPND；Beach，1942)，可致所有年龄发生急性，通常是致死性疾病，以出现呼吸道和神经症状为特征。
中发型新城疫(MND； Beaudette and Black，1946)，呼吸系统或神经系统疾病的低致病性形式。死亡仅见于幼雏。
缓发型新城疫(LND；Hitchner 和Johnson，1948)，轻度或不显性的呼吸道疾病。
无症状型或缓发嗜肠型新城疫 (LEND；Lancaster，1981)，主要是肠道感染。
速发嗜内脏型新城疫(VVND；Doyle，1927)，可致所有年龄的鸡发生最急性或急性、致死性疾病。通常见有消化道出血性病变。
速发嗜肺脑型新城疫(VPND；Beach，1942)，可致所有年龄发生急性，通常是致死性疾病，以出现呼吸道和神经症状为特征。
中发型新城疫(MND； Beaudette and Black，1946)，呼吸系统或神经系统疾病的低致病性形式。死亡仅见于幼雏。
缓发型新城疫(LND；Hitchner 和Johnson，1948)，轻度或不显性的呼吸道疾病。
无症状型或缓发嗜肠型新城疫 (LEND；Lancaster，1981)，主要是肠道感染。
背景资料
The entry of Newcastle disease virus (NDV), a prototype paramyxovirus, is directed by two virion glycoproteins, the hemagglutinin-neuraminidase (HN) protein and the fusion (F) protein . HN protein, the virus attachment protein, binds to sialic acid-containing receptors, and F protein mediates membrane fusion. In contrast to many viral fusion proteins, paramyxovirus F proteins do not require the acid pH of endosomes to activate fusion activity. As a consequence, infected cells expressing both attachment proteins and F proteins can fuse with adjacent cells to form multinuclear cells, or syncytia, a process that is assumed to mimic virus-cell fusion .
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靶标
基因名
HN
蛋白名
Hemagglutinin-neuraminidase
亚基
Homotrimer of disulfide-linked F1-F2.
亚细胞定位
Virion membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. Host cell membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
翻译后修饰
The inactive precursor F0 is glycosylated and proteolytically cleaved into F1 and F2 to be functionally active. The cleavage is mediated by cellular proteases during the transport and maturation of the polypeptide (By similarity). {ECO:0000250}.
相似性
Belongs to the paramyxoviruses fusion glycoprotein family.
功能
Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (F protein) probably interacts with HN at the virion surface. Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between cell and virion membranes. Later in infection, F proteins expressed at the plasma membrane of infected cells could mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis.
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