PRMT5 Rabbit pAb (一抗) | Bioss
货号：bs-6992R
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概述
产品编号
bs-6992R
产品类型
宠物抗体、农牧业/家禽抗体
英文名称
PRMT5 Rabbit pAb
中文名称
组蛋白精氨酸甲基转移酶5抗体
英文别名
HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs; ANM5_HUMAN; PRMT5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog (SKB1 homolog | SKB1Hs); 2.1.1.320; ANM5_MOUSE; Shk1 kinase-binding protein 1 homolog (SKB1 homolog);
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human PRMT5: 201-300/637
亚型
IgG
性状
Liquid
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
70 kDa
检测分子量
67 kDa
浓度
1mg/ml
储存液
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
精氨酸甲基转移酶，既能催化omega-N单甲基精氨酸（MMA）和对称二甲基精氨酸（sDMA）的形成，又能优先催化MMA的形成。特异性介导小核核糖核蛋白smd1（SNRPD1）和smd3（SNRPD3）中精氨酸残基的对称二甲基化；这种甲基化是snRNP核心粒子组装和生物生成所必需的。甲基化SUPT5H并可能调节其转录延伸特性。髓鞘碱性蛋白（MBP）精氨酸残基的单酸和二甲基化。可能在细胞因子激活的转导途径中发挥作用。负调控细胞周期素E1启动子活性和细胞增殖。在生殖细胞发育过程中甲基化组蛋白H2A和H4'Arg-3'。甲基化组蛋白H3'Arg-8'，可能抑制转录。甲基化Piwi蛋白（PIWIL1，PIWIL2和PIWIL4），Piwi蛋白甲基化是与包含Tudor结构域的蛋白质相互作用和随后定位到减数分裂的细微差别所必需的。
背景资料
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human, Mouse | Rat, Rabbit, Pig, Cow, Dog, Horse | 1:500-2000 |
| IHC-P | Human, Mouse | Rat, Rabbit, Pig, Cow, Dog, Horse | 1:100-500 |
| IHC-F | Human, Mouse | Rat, Rabbit, Pig, Cow, Dog, Horse | 1:100-500 |
| IF | Human, Mouse | Rat, Rabbit, Pig, Cow, Dog, Horse | 1:100-500 |
交叉反应
交叉反应: Human, Mouse (predicted: Rat, Rabbit, Pig, Cow, Dog, Horse)
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靶标
基因名
PRMT5
蛋白名
Protein arginine N-methyltransferase 5
亚基
Forms, at least, homodimers and homotetramers. Interacts with PRDM1. Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4. Interacts with LSM11, PRMT7 and SNRPD3. Interacts with COPR5/C17orf79; promoting its recruitment on histone H4. Interacts with RPS10. Interacts with EGFR; methylates EGFR and stimulates EGFR-mediated ERK activation. Interacts with BRAF and with active RAF1. Interacts with HOXA9.
亚细胞定位
Cytoplasm. Nucleus.
组织特异性
Ubiquitous.
翻译后修饰
Disulfide bonds and non-covalent association mediate homooligomers formation.
相似性
Belongs to the protein arginine N-methyltransferase family.
功能
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9.
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