Nestin Mouse mAb (一抗) - WB,IHC-P,IHC-F,IF | Bioss
货号：bsm-60784M
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概述
产品编号
bsm-60784M
产品类型
病理级抗体
英文名称
Nestin Mouse mAb
中文名称
巢蛋白/神经上皮干细胞蛋白单克隆抗体
英文别名
Nbla00170; ESTM46; Ifaprc2; Marc2; RC2; NEST_HUMAN; NES; NEST_MOUSE; NEST_RAT; nestin
抗体来源
Mouse
免疫原
KLH conjugated synthetic peptide derived from human Nestin
亚型
IgG2a, κ
性状
Liquid
纯化方法
affinity purified by Protein G
克隆类型
Monoclonal
克隆号
E2G4
理论分子量
178 kDa
检测分子量
240-300 kDa
浓度
1mg/ml
储存液
PBS, Glycerol, BSA.
研究领域
Cancer > Invasion/microenvironment > Angiogenesis
Cancer > Tumor biomarkers > Tumor antigens
Cardiovascular > Angiogenesis > Angiogenic Factors
Developmental Biology > Lineage specification > Ectoderm
Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments
Neuroscience > Cell Type Marker > Neural Stem Cell marker
Neuroscience > Neurology process > Neurogenesis
Signal Transduction > Antibodies > nestin
Stem Cells > Lineage Markers > Ectoderm
SWISS
Gene ID
保存条件
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
产品介绍
神经干细胞标志物
在动物体早期发育过程中，中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白，它在哺乳动物神经前体细胞中表达较高，目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律，将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统干细胞及神经上皮细胞。
背景资料
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
产品应用
| 应用 | 已检合格种属 | 预测种属 | 推荐稀释比例 |
|---|---|---|---|
| WB | Human | 1:500-1000 | |
| IHC-P | Human | 1:100-500 | |
| IHC-F | Human | 1:100-500 | |
| IF | Human | 1:100-500 |
交叉反应
交叉反应: Human
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靶标
基因名
NES
蛋白名
Nestin
亚基
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
亚细胞定位
cytoplasm
组织特异性
CNS stem cells.
翻译后修饰
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
相似性
Belongs to the intermediate filament family.
功能
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
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