TUBA4A | alpha-Tubulin Mouse mAb (一抗) - WB,IHC-P,IHC-F,IF,Flow-Cyt,ICC/IF | Bioss

bsm-33039M

宠物抗体、模式动物抗体、农牧业/家禽抗体

TUBA4A | alpha-Tubulin Mouse mAb

微管蛋白α/Tubulin α/α-tubulin单克隆抗体

B-ALPHA-1; LIS3; TUBA3; Tuba-1; Tuba1; TBA1A_HUMAN; TUBA1A; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; 3.6.5.-; TBA1A_MOUSE; Alpha-tubulin 1; Alpha-tubulin isotype M-alpha-1; Tubulin alpha-1 chain; TBA1A_RAT; tubulin alpha 1a; tubulin, alpha, brain-specific

Mouse

Recombinant human alpha Tubulin Protein

IgG

Size : 50ul/100ul/200ul
Liquid

Size : 200ug (PBS only)
Lyophilized
Note: Centrifuge tubes before opening. Reconstitute the lyophilized product in distilled water. Optimal concentration should be determined by the end user.

affinity purified by Protein G

Monoclonal

10F11

50 kDa

50 kDa

1mg/ml

Size : 50ul/100ul/200ul
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Size : 200ug (PBS only)
0.01M PBS (pH7.4).

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

TUBA4A

Tubulin alpha-4A chain

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Cytoplasm, cytoskeleton.

Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).

Belongs to the tubulin family.

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain